Our zebrafish facility created in 2009, is devoted to research on zebrafish for scientists working in various domains including neurosciences, development, and human diseases.

What services ?

  • Production of zebrafish embryos and adults
  • Zebrafish husbandry
  • Morpholino injection
  • Calcium imaging
  • Support to neuroscience projects
  • Support of imaging of microglia and neurons

Why use zebrafish model ?

Zebrafish (Danio rerio) has become a powerful and versatile animal model for translational research on human neurological disorders. Beyond of its remarkable optical transparency, this small vertebrate exhibit a profound resemblance with the human brain. Their relatively simple nervous system permit real-time neurological imaging, and Ca2 imaging. The zebrafish embryos are quite malleable to genetic manipulation by morpholino antisense oligonucleotide, mRNAs, transgenes and genome editing techniques like CRISPR-Cas9, and TALENs technologies.

Expertise

In vivo imaging of microglia
Calcium imaging
Morpholinos injection
CRISPR-Cas 9 technologies
Confocal and two-photon microscopy
Image analysis: ImageJ, Imaris

Equipement

Housting Area
Wild type, transgenic, and mutants lines
Daily artemia feeding
Daily paramecia feeding
High Resolution Melt Analysis (HRMA) Embryos and adults genotyping
Microinjectors
Stereomicroscopes

Team

Nadia Soussi-Yanicostas– Scientific manager
Constantin Yanicostas – Technical manager
Christiane Romain and Olivier Bar – Operational manager

Contact

Nadia Soussi-Yanicostas
Tel : 01 40 03 19 31
Email : nadia.soussi@inserm.fr
INSERM UMR1141- NeuroDiderot -Université de Paris
Hôpital Robert Debré
48 bd Sérurier,
75019 Paris – France

Equipments

Zebrafish embryos

 

Transgenic lines

 

Automated microinjection device

 

Fish housing facility

 

Publications with zebrafish

2021
– Alexandre Brenet , Rahma Hassan-Abdi, and Nadia Soussi-Yanicostas.
Bixafen, a succinate dehydrogenase inhibitor fungicide, causes microcephaly and motor neuron axon defects during development
Chemosphere, Chemosphere. 2021 Feb;265:128781. doi: 10.1016/j.chemosphere.2020.128781.

2020
– Somkhit J, Loyant R, Brenet A, Hassan-Abdi R, Yanicostas C, Porceddu M, Borgne-Sanchez A, Soussi-Yanicostas N.
A fast, simple and affordable technique to measure oxygen consumption rates in living zebrafish embryos.
Zebrafish. 2020 , May 4. doi: 10.1089/zeb.2020.1878.
– Brenet A, Somkhit J, Hassan-Abdi R, Yanicostas C, Igert A, Saurat D, Taudon N, Dal-Bo G, Nachon F, Dupuis , and Soussi-Yanicostas N.
Preclinical zebrafish model for organophosphorus intoxication: neuronal hyperexcitation, behavioral abnormalities and subsequent brain damages.
Scientific Reports, 2020 Nov 5;10(1):19228. doi: 10.1038/s41598-020-76056-8.

2019
– Brenet A, Hassan-Abdi R, Somkhit J, Yanicostas C, Soussi-Yanicostas N.
Defective Excitatory/Inhibitory Synaptic Balance and Increased Neuron Apoptosis in a Zebrafish Model of Dravet Syndrome.
Cells. 2019; 8(10):1199. doi: 10.3390/cells8101199.
– Hassan-Abdi R, Brenet A, Bennis M, Yanicostas C, Soussi-Yanicostas N.
Neurons Expressing Pathological Tau Protein Trigger Dramatic Changes in Microglial Morphology and Dynamics.
Front Neurosci. 2019;13. doi: 10.3389/fnins.2019.01199.
– Mairesse J, Zinni M, Pansiot J, R Hassan-Abdi , C Demene , M Colella , C Charriaut-Marlangue , A Novais , M Tanter , S Maccari , P Gressens , D Vaiman ,  O Baud*, N Soussi-Yanicostas*.  * last co-authors.
Oxytocin receptor agonist reduces perinatal brain damage by targeting microglia.
Glia. 2019;67(2):345-359. doi: 10.1002/glia.23546.
– Van Steenwinckel J, Schang AL ,  Krishnan M,  Degos V,  Delahaye-Duriez A,  Bokobza,  Csaba Z , Verdonk F ,  Montané A, Sigaut S, Hennebert O, Lebon S,  Schwendimann L, Le Charpentier T, Hassan-Abdi R, Ball G,  Aljabar P ,  Saxena A,  Holloway R,  Birchmeier W,  Baud O,  Rowitch D,  Miron V,  Chretien C ,  Leconte C ,  Besson V,  Petretto E,  Edwards D ,  Hagberg E ,  Soussi-Yanicostas N,  Fleiss B,  Gressens P Decreased microglial Wnt/β-catenin signalling drives microglial pro-inflammatory activation in the developing brain.
Brain. 2019;142(12):3806-3833. doi:10.1093/brain/awz319.

2018
– Samarut É, Swaminathan A, Riché R, Liao M, Hassan-Abdi R, Renault S, Allard M, Dufour L, Cossette P, Soussi-Yanicostas N, Drapeau P.
γ‐Aminobutyric acid receptor alpha 1 subunit loss of function causes genetic generalized epilepsy by impairing inhibitory network neurodevelopment.
Epilepsia. 2018;(September):2061-2074. doi:10.1111/epi.14576.
– Swaminathan A, Hassan-Abdi R, Renault S, Siekierska A, Riché R, Liao M, de Witte P, Yanicostas C, Soussi-Yanicostas N, Drapeau P, E Samarut.
Non-canonical mTOR-Independent Role of DEPDC5 in Regulating GABAergic Network Development.
Curr Biol. 2018;28:1924-1937. doi: 10.1016/j.cub.2018.04.061.
– Alavi Naini SM, Yanicostas C, Hassan-Abdi R, Blondeel S, Bennis M, Weiss RJ, Tor Y, Esko JD, Soussi-Yanicostas N.
Surfen and oxalyl surfen decrease tau hyperphosphorylation and mitigate neuron deficits in vivo in a zebrafish model of tauopathy.
Transl Neurodegener. 2018;7(1). doi:10.1186/s40035-018-0111-2.

2016
– Lebcir A, Hassan-Abdi R, Yanicostas C, Soussi-Yanicostas N.
A Rapid and Efficient Method of Identifying G0 Males with Mosaic Germ Line Cells.
Zebrafish. 2016 Dec;13(6):535-536. DOI: 10.1089/zeb.2016.1363.

2015
– Alavi Naini* SM, Sepulveda-Diaz* JE, Huynh* MB, Ouidja MO, Yanicostas C, Chantepie S, Villares J, Lamari F, Jospin E, van Kuppevelt TH, Mensah-Nyagan AG, Raisman-Vozari* R, Papy-Garcia* D, Soussi-Yanicostas* N.
HS3ST2 expression is critical for the abnormal phosphorylation of tau in Alzheimer’s disease-related tau pathology.
Brain. 2015;138(Pt 5):1339-54. *Co-first and last authors. doi: 10.1093/brain/awv056.

2013
– Ghoumid J, Drevillon L, Alavi-Naini SM, Bondurand N, Rio M, Briand-Suleau A, Nasser M, Goodwin L, Raymond P, Yanicostas C, Goossens M, Lyonnet S, Mowat D, Amiel J, Soussi-Yanicostas N, Giurgea I.
ZEB2 zinc-finger missense mutations lead to hypomorphic alleles and a mild Mowat-Wilson syndrome.
Hum Mol Genet. 2013, 1;22(13):2652-61. doi: 10.1093/hmg/ddt114.

2012
Martin E, Yanicostas C, Rastetter A, Naini SM, Maouedj A, Kabashi E, Rivaud-Péchoux S, Brice A, Stevanin G, Soussi-Yanicostas N.
Spatacsin and spastizin act in the same pathway required for proper spinal motor neuron axon outgrowth in zebrafish.
Neurobiol Dis. 2012 Dec;48(3):299-308. doi: 10.1016/j.nbd.2012.07.003.

Ayari B, Landoulsi A, Soussi-Yanicostas N.
Localization and characterization of kal 1.a and kal 1.b in the brain of adult zebrafish (Danio rerio).
Brain Res Bull. 2012, Jul 1;88(4):345-53. doi: 10.1016/j.brainresbull.2012.03.006.

2010
Ayari B, El Hachimi KH, Yanicostas C, Landoulsi A, Soussi-Yanicostas N.
Prokineticin 2 expression is associated with neural repair of injured adult zebrafish telencephalon.
J Neurotrauma. 2010 May; 27(5):959-72.

2009
Yanicostas C, Herbomel E, Dipietromaria A, Soussi-Yanicostas N.
Anosmin-1a is required for fasciculation and terminal targeting of olfactory sensory neuron axons in the zebrafish olfactory system.
Mol Cell Endocrinol. 2009 Nov 27;312(1-2):53-60. doi: 10.1016/j.mce.2009.04.017.

2008
Yanicostas C, Ernest S, Dayraud C, Petit C, Soussi-Yanicostas N.
Essential requirement for zebrafish anosmin-1a in the migration of the posterior lateral line primordium.
Dev Biol. 2008 Aug 15;320(2):469-79. doi: 10.1016/j.ydbio.2008.06.008.

2007
Ernest S, Guadagnini S, Prévost MC, Soussi-Yanicostas N.
Localization of anosmin-1a and anosmin-1b in the inner ear and neuromasts of zebrafish.
Gene Expr Patterns. 2007 Jan;7(3):274-81.

Patents using zebrafish model

Soussi-Yanicostas N, Yanicostas C, Alavi-Naini MS. 2011. International patent (deposit number: PCT/EP2012106523). Materials and methods for the treatment of tauopathies. Ce brevet a été étendu aux Etats-Unis en Juin 2016 (N° de dépôt : 15/196,180).

Papy-Garcia D, Huyn B, Soussi-Yanicostas N, et al.  2012. European patent (deposit number: 12300005414.0-2107). Method of diagnosis, pronostic or treatment of neurodegenerative diseases. Ce brevet a été étendue aux Etats-Unis (Date de dépôt 01/06/2016, N° Dépôt 15/196,180), Déposant: SATT Ile-de-France Innov.

Contact

Nadia Soussi-Yanicostas
Tel : 01 40 03 19 31
Email : nadia.soussi@inserm.fr
INSERM UMR1141- NeuroDiderot -Université de Paris
Hôpital Robert Debré
48 bd Sérurier,
75019 Paris – France